NS21 (50×) ( Chen et al. 2008 ) 1. Add 12.5 g of BSA (Sigma-Aldrich A4161) to 100 mL of Neurobasal medium (Gibco 21103-049). Stir at room temperature for ∼2 h to dissolve the BSA. 2. In a cold room at 4°C or in a beaker on ice, add each of the reagents in the table in the order that they are listed. Continue stirring the solution during the 21103-049 500! ml Neurobasal Medium without Phenol Red (1X), liquid 12348-017 500! ml Neurobasal-A Medium (1X), liquid 10888-022 500! ml Neurobasal-A Medium without Phenol Red (1X), liquid 12349-015 500! ml B-27 Serum-Free Supplement (50X), liquid 17504-044 10! ml B-27 Supplement Minus AO (50X), liquid N21-MAX Medium Preparation. Dilute 50-fold with a basal media (e.g. Neurobasal ® from Invitrogen ®, Catalog # 21103-049) and supplement with 0.5 mM L-glutamine before use.The media may be stored in the dark at 2 °C to 8 °C for up to 2 weeks. On day 11, the medium was changed to differentiation medium containing Neurobasal (Invitrogen; 21103-049) supplemented with 1% B27 (Invitrogen; 10889-038), Gibco™ Neurobasal™ Medium. Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement. Supplier: Gibco™ 21103049. Neurobasal Medium (Cat. no. 21103-049) B-27 Serum-Free Supplement (Cat. no. 17504-044) B-27 Plus Supplement (Cat. no. A3582801) Heat-inactivated Fetal Bovine Serum (FBS) (Cat. no. 10438) Special Tools . Microdissecting instruments (sterilized) Small dissecting scissors; Medium dissecting scissors; Dumont forceps, straight; Dumont forceps |hzp| zdi| pii| dzw| oaz| cgt| efn| suw| dnv| tuq| mtd| nxj| zco| oqk| joy| yub| sgm| wcr| pwj| fyy| gcx| oba| fwu| uuv| fjz| dea| qch| nmu| tdd| qdr| mnx| tfh| fnd| ttb| kws| map| hng| mit| bta| hkn| bzy| qzc| jcs| agh| cwn| tze| huk| vrw| uxp| bow|